
Current in-vitro models of metastasis fail to recapitulate the critical cell–matrix interactions involving the pre-metastatic extracellular matrix (ECM) of liver, lung, and bone tissues. Tissue-specific ECM substrates have biochemical and mechanical features that enable disease-relevant in-vitro modeling of metastatic processes in bone, liver, and lung tissue microenvironments and are compatible with 3D applications and assays.